Year One of a Test of Biological Fungicides in Strawberries.

Oct 9, 2013

This is simply a summary of one year of biological fungicide work in strawberries in 2012-2013 and should not be understood as a recommendation to use any of these products.  This investigation is will continue into 2013-2014 and will serve to confirm and adjust the work here.

Introduction: A number of biological fungicides registered for use in strawberries have not been thoroughly tested through empirical studies to give guidance to growers on their efficacy and use.

 

Materials and Methods:

Table 1 below is an outline of materials tested in 2012-2013.

The field involved in the study was organically farmed and had a tested infestation of Verticillium at the average level of 25 microsclerotia per treatment replicate.  Grower standard was managed as per grower practice, that is to say normal irrigation, fertility and pest management practices were applied.  This grower standard did not include any sort of biological fungicide either by dip or injection through the drip tape during the season.

Plot was replicated three times and each replicate consisted of at one bed of 180 feet in length. 

Application of the materials took place as indicated in the table below:

 

Table 1. List of treatments.

Test   Material

Application/   Use

Dazitol

6.25 gal/A   applied 3-5 days prior to planting

Biotam +   Serenade Soil

5 lbs/A   pre-plant application 3-5 days prior to planting followed by Serenade Soil @   4 qt/A (10 days after planting, and then Serenade Soil @ 2 qt /A applied   monthly after planting.  Second   application of Biotam 5 lbs/A and Serenade Soil @ 4 qt/A in February.

Serenade   Soil

Serenade   Soil @ 4 qt/A (10 days after planting, and then Serenade Soil @ 2 qt /A   applied monthly after planting.

Serenade   Soil

Serenade   Soil @ 4 qt/A (10 days after planting, and then Serenade Soil @ 4 qt /A   applied every 60 days after planting.

Actinovate   Rate 1

(1) 3 oz   per 100 gal root dip at planting.

(2)   followed by 6 oz/A pre-plant in drip tape

(3)   followed by 3 oz/A every 30 days in drip tape

 

Actinovate   Rate 2

(1) 3 oz   per 100 gal root dip at planting.

(2)   followed by 6 oz/A pre-plant in drip tape

(3)   followed by 6 oz/A every 30 days in drip tape

 

SoilGuard

Apply at   planting as root dip or planting furrow drench at 5 lb/ A and again through   drip tape every 4-6 weeks through harvest.

Double   Nickel 55

Apply at   planting at 1 qt /A (1/2 lb/A powder) as root dip or planting furrow drench   and again through drip tape every 4-6 weeks through harvest.

Terra   Clean 5.0

(1) 128 fl   oz/100 gal drench

(2) 2   gal/A drip applied at planting

(3) 1   gal/A drip applied 10 days post plant and 28 days post plant

(4) 1   gal/A drip applied 60, 90 and 120  days   post plant

Terra Clean 5.0 + Serenade Soil

(1) 128 fl   oz Terra Clean /100 gal drench + Serenade Soil @ 6 qt/ A soil drench

(2) 2   gal/A Terra Clean drip applied at planting followed by SS @ 3 qt /A

(3) 1   gal/A Terra Clean + 3 qt /A Serenade Soil drip applied 10 days post plant and   28 days post plant

(4) 1   gal/A Terra Clean + Serenade Soil @ 3 qt/ A drip applied 60, 90 and 120 days   post plant

Tainio

(1)   Spectrum @ 50 g / A + Pepzyme C @ 12.5 oz/A 2- 3 days preplant

(2)   Biogenesis @ 1 lb/A+ Pepzyme C @ 12.5 oz/A as plant dip

(3)   Pepzyme C monthly through drip tape

(4) Micro   5000 @ 2.66 oz/A at 2 leaf stage foliar

Plant dips were made by suspending the requested rate of fungicide in approximately twenty gallons of water and submerging and soaking about a half a box of strawberry transplants (about 500 plants) thoroughly and then distributing to planting crews for transplant.  Note that in the case of the two Actinovate treatments, plants were held for one night to prior to transplant, ostensibly to establish the organism on the plant roots.

Drip applications were made with a portable pump injecting each fungicide.  Each application normally was preceded by filling the drip tape with clear water, injecting the mix and then further pumping in clear water to make sure the fungicide had moved well out of the drip tape.

 

Application dates:

Preplant application – 11/3/2012

            Dazitol

            Terra Clean 5.0

            Terraclean 5.0 + Serenade Soil

            Tainio Spectrum + Pepzyme C

            Biotam

 

Root dip + one overnight hold – 11/7/2012 (planted November 8)

            Actinovate rate 1

            Actinovate rate 2

Root dip and immediate planting – 11/8/2012

            Double Nickel 55

            Soilguard

            Biogenesis + Pepzyme C

 

10 days post – plant – 11/20/2012

            Serenade 4 qt

            Serenade 2 qt

 

Monthly applications (12/11/2012, 1/16/2013, 2/26/2013, 3/27/2013, 4/30/2013,  and 6/13/2013)

 

Terra Clean 5.0

Terraclean 5.0 + Serenade Soil

Tainio Spectrum + Pepzyme C

Serenade 4 qt

Serendade 2 qt

Actinovate rate 1

Actinovate rate 2

Double Nickel 55

Biotam applied 2/26/2013; Serenade 4 qt per acre applied other dates in treatment

Soilguard

 

Several dying plants from different areas of the test plots were sampled in July to confirm that Verticillium was the cause of plant death.

As a gauge of plant vigor from each treatment, strawberry plant diameters were measured February 13, and April 13.  Measurements were in centimeters and from twenty plants in each plot.

With the beginning of fruit ripening, fruit harvest was done weekly in each treatment replicate.   On each pick date, fruit from each plot was weighed and counted.

 

Results:

 

 

Plant D cm

2/13/2013

Plant D

cm

4/13/2013

Yield to

5/14/2013

April Total   Yield g/plot

Actinovate   r1

14.05a

16.25a

3894.00a

2175.00ab

Actinovate   r2

13.70ab

16.42a

3818.33a

2428.00a

Biotam

11.47b

15.77a

2176.67b

1377.00b

Dazitol

11.70b

17.70a

2505.67ab

1568.00ab

Double   Nickel 55

12.65ab

15.92a

3084.00ab

1637.00ab

Serenade   4 qt +2 qt

11.57b

16.65a

2853.00ab

1471.67b

Serenade   4 qt +4 qt

12.42ab

16.02a

2621.00ab

1459.67b

Soilguard

11.85b

16.78a

2993.33ab

1652.33ab

Tainio

12.93ab

16.95a

3454.00ab

2075.33ab

Terraclean

11.50b

16.78a

2284.00b

1406.67b

Terraclean   + Serenade

12.47ab

17.11a

2656.33ab

1559.67ab

Untreated   grower standard

11.70b

17.90a

2145.00b

1189.00b

Means followed by same letter do not significantly differ (P=.05, Student-Newman-Keuls)

 

 

May Total   Yield g/plot

June Total   Yield g/plot

July Total   Yield g/plot

Aug Total   Yield g/plot

Total

Yield g/plot

Actinovate   r1

3741.67a

2791.00a

1902.33a

140.64a

16820.67a

Actinovate   r2

3069.67a

3237.67a

2812.33a

149.35a

17201.34a

Biotam

2254.00a

3251.33a

3402.00a

503.14a

14468.33a

Dazitol

2514.67a

3075.33a

3049.33a

269.16a

14625.00a

Double   Nickel 55

3038.67a

2837.67a

2573.67a

203.64a

15052.67a

Serenade   4 qt +2 qt

3244.67a

3287.00a

3623.67a

371.49a

16887.67a

Serenade   4 qt +4 qt

2959.67a

3617.67a

3236.33a

362.02a

16157.00a

Soilguard

3212.33a

3331.67a

2887.00a

359.45a

16352.33a

Tainio

3409.00a

3687.33a

3190.33a

170.27a

18145.00a

Terraclean

2496.67a

2997.00a

2750.00a

125.01a

13681.33a

Terraclean   + Serenade

2544.00a

2810.00a

2230.00a

191.78a

13454.33a

Untreated   grower standard

2812.00a

4019.33a

3800.00a

306.15a

16150.33a

Means followed by same letter do not significantly differ (P=.05, Student-Newman-Keuls)

 

Plant diameters measured on February 13, 2013, were significantly larger in the low rate of Actinovate than the other treatments with the exception of the high rate of Actinovate, Tainio and Double Nickel 55.

Both rates of Actinovate realized higher fruit yield than all other treatments except for the Dazitol, in cumulative fruit yield, which included six weekly harvests, up to May 14, 2013.  No further differences were realized between May and August.

As noted above, this field has a very high infestation of Verticillium and all plots began to experience pronounced plant dieback in June, with some 60-80% remaining alive or declining and by end of July, nearly all plants in all replicate plots had died.   There was a trend for plants which had produced larger amounts of fruit in April and May to experience lesser fruit production lesser vigor and earlier dieback as the season progressed.

 

Conclusion

The results of this trial are encouraging.  In the early part of the season up through the middle of May, several treatments had significantly higher amounts of fruits harvested than the grower standard.  Nonetheless, none of the treatments provided sufficient protection to the plants to prevent an almost complete die out of the plants by August, effectively ending the season.

 

 


By Mark Bolda
Author - Farm Advisor, Strawberries & Caneberries
By Monise Sheehan
Author